A real-time polymerase chain reaction (Real-Time PCR) is a laboratory technique of molecular biology. It is also known as quantitative polymerase chain reaction. The name has been given as it is based on the polymerase chain reaction (PCR). The changes in The PCR process take place for approx 25 – 50 times. It is by recurring nature. The temperatures and the timings used for each cycle depend on a wide variety of parameters. There are two ways used commonly. It is done basically to detect PCR products in real- time. The acronym "RT-PCR" denotes reverse transcription polymerase chain reaction.