Proteins play a vital role in biological processes, and amino acids are building blocks are the building blocks of it. Amino acids make up the large quotient of our cells, muscles, and tissues. Amino acids also are important for the transport and storage of nutrients; they also have an influence on the functioning of glands, and Organs. 21 amino acids can form proteins in the human body. The amino acid disorder can lead to clinical sign or symptom. Amino acid disorder if not diagnosed and treated properly can lead to mental retardation or death. Amino acid analysis/testing refers to quantitative analysis of either free amino Acids or amino acids released from proteins from proteins.