Mumps is caused by RNA Virus called Paramyxoviridae. Mumps outbreak happened in UK, USA, and Canada. A person who was partially vaccinated got affected by this disease. This test is essential when the following symptoms show up on an individual like, Mild to high fever, swelling of the parotid gland in children or chewing causing pain in adults, loss of appetite, abdominal pain, a cough and cold in children. PCR (Polymerase Chain Reaction)identifies this disease by Reverse Transcriptase (RT enzyme) infusion method in which RNA helps to replicate the allergy-causing virus to make it visible.