IHC Marker (Eight Markers) Test

In order to determine the tissue distribution of an antigen of interest in health and disease, Immunohistochemistry (IHC) is an important application of monoclonal as well as polyclonal antibodies to look forward to. IHC is widely used for diagnosis of cancer. This article deals with the various applications of IHC in diagnosis of diseases, with IHC playing an important role in diagnostic and research laboratories. Examples of some commonly used markers include: BrdU: used to identify replicating cells. Used to identify tumors as well as in neuroscience research. Cytokeratins: used for identification of carcinomas but may also be expressed in some sarcomas. CD15 and CD30 : used for Hodgkin's disease, Alpha fetoprotein: for yolk sac tumors and hepatocellular carcinoma, CD117 (KIT): for Preparation of the sample is critical to maintain cell morphology, tissue architecture and the antigenicity of target epitopes. This requires proper tissue collection, fixation and sectioning. A solution of paraformaldehyde is often used to fix tissue, but other methods may be used. and acute lymphoblastic leukemia, Prostate specific antigen (PSA): for prostate cancer, estrogens and progesterone receptor (ER & PR) staining are used both diagnostically (breast and gyn tumors) as well as prognostic in breast cancer and predictive of response to therapy (estrogen receptor), Identification of B-cell lymphomas using CD20 and Identification of T-cell lymphomas using CD3.

A person can approach to a closest lab without any special preparation. But it is advised to consult with your doctor first if you are under any sort of medication or allergic to something. The doctor after consultation can write special instruction as to how to and when to conduct this test.

IHC is used for disease diagnosis, biological research, and in drug development. For example, using specific tumor markers, physicians use IHC to diagnose if a tumor is benign or malignant, to determine its stage and grade, and to identify the cell type and origin of a metastasis in order to find the site of the primary tumor. A variety of other non-neoplastic diseases and conditions are diagnosed using IHC as a primary tool or as a confirmatory procedure.

Preparation of the sample is critical to maintain cell morphology, tissue architecture and the antigenicity of target epitopes. This requires proper tissue collection, fixation and sectioning. A solution of paraformaldehyde is often used to fix tissue, but other methods may be used.