IHC MPO with interpretation Test

MPO is usually stored in a certain type of white blood cell. These cells release MPO when blood vessels are injured or become inflamed. Myeloperoxidase (MPO) is an unequivocal marker of myeloid differentiation which is routinely detected using cytochemistry (CC), flow cytometry (FC) and immunohistochemistry (IH).

Immunohistochemistry is a more sensitive marker for the detection of myeloperoxidase in acute myeloid leukemia compared with flow cytometry and cytochemistry. IHC takes its name from the roots 'immuno', in reference to antibodies used in the procedure, and 'histo,' which means “tissue”. Immunohistochemistry is also widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.

The preparation of the test to detect Myeloperoxidase is not very hectic. The patient’s MPO level will be checked with a simple blood test, for the purpose of the test the patient doesn’t need to follow any special instructions prior to the test. The test can be done at any time of the day. During the test the patient will not have to fast or stop taking any medications. But, if the patient is having blood drawn for other tests, the patient may need to follow special instructions not related to the MPO test.

Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. Immunohistochemistry is also widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue. Immunohistochemistry can also be used for a more general protein profiling, provided the availability of antibodies validated for immunohistochemistry. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

The preferred specimen for the test is formalin-fixed paraffin embedded tissue in an IHC specimen transport kit. The alternative specimen that can be used for the test includes Five 4-micron unstained slides ,Fixed tissue and Tissue in neutral buffered formalin Preparation of the sample is critical to maintain cell morphology, tissue architecture and the antigenicity of target epitopes. This requires proper tissue collection, fixation and sectioning. The specimen should be maintained at room temperature. If the specimen is frozen then it is not acceptable for the test.