Periodic acid–Schiff (PAS) is a staining method used to spot polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids, and mucins in tissues. The reaction of periodic acid oxidizes the vicinal diols in these sugars, generally breaking up the bond among two nearby carbons not tangled in the glycosidic linkage or ring closure in the ring of the monosaccharide units that are parts of the stretched polysaccharides, and forming a pair of aldehydes at the two free tips of each cracked monosaccharide ring. The oxidation condition has to be appropriately regulated so as to not oxidize the aldehydes more. These aldehydes then respond with the Schiff reagent to give a purple-magenta color. A suitable basic stain is frequently used as a counterstain. PAS diastase stain (PAS-D) is PAS stain used in amalgamation with diastase, an enzyme that cessations down glycogen. Alcian blue/periodic acid–Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step.
Following preparations are required to conduct the test.
Periodic Acid Solution :
§ Periodic Acid: 1 gram
§ Distilled water: 100 ml
Schiff’s Reagent :
§ Fuchsin Basic: 1 gm
§ Distilled water: 100 ml
§ Sodium metabisulphite: 2 gm
§ Conc. HCl : 2 ml
§ Charcoal activated : 0.3 gm
Dissolve basic fuchsin in hot water, cool at 50°C and filter. Add sodium metabisulphite and HCl.
Store at dark room at room temperature overnight. Add charcoal, shake this for one minute and
then filter.
PAS stain is generally used to focus the molecules (structures) with a high percentage of
carbohydrate content such as glycogen, glycoproteins, and proteoglycans typically found in
connective tissue, glycocalyx, and basal laminae. PAS staining can be used to support the
diagnosis of numerous medical conditions such as:
§ Glycogen storage disease (vs. other storage diseases)
§ Adenocarcinoma which often secretes mucin
§ Paget’s disease of breast
§ Alveolar soft part sarcoma
§ Staining macrophages in Whipple’s disease
§ Erythroleukemia, Leukemia of immature RBCs
§ Fungal infection (cell wall stain magenta)
1. Bring sections to distilled water.
2. Treat them with periodic acid for 5 minutes.
3. Rinse it well in distilled water.
4. Cover with Schiff’s reagent for 5-15 minutes.
5. Wash it in running tap water for 5-10 minutes
6. Counterstain with Herri’s hematoxylin for around 15 seconds.
7. Differentiate with acid alcohol and bluing as always.
8. Wash in tap water.
9. Rinse in increasing concentration of alcohol
10. Clear in xylene and mount as usual.
| Type | Gender | Age-Group | Value |
|---|---|---|---|
|
Periodic Acid Schiff Stain Bone Marrow
|
Unisex
|
All age groups
|
Stain Color is Block like positivity is observed in acute lymphoblastic lymphoma. Magenta pink color is seen in positive staining
|
Supplements are just a way to add vital nutrients in your daily nutritional intake, but are not the only solution. First of all, it is important to note that your sex life does not depend on supplements. Various other factors can both positively and negatively affect your sex life. Exercise, weight and medications you take can have both adverse and positive effects. Try to improve your sex life by altering these aspects before you use supplements. Nevertheless, here are some supplements which may be beneficial for your sex life.
When it comes to maintaining proper penis health, most men have a very good idea of what to do. They know all the basics of penis care, from taking care to wash properly to regularly inspecting the manhood to applying a high-quality penis health crème. But in the heat of the moment, when sexual fun is getting hot and heavy, a man might make a silly mistake. Those sexual mistakes can compromise penis health in a serious way.
Not sure what sexual mistakes might be a problem? Here's a list of the biggest issues a man might find himself doing when things are hot - and might regret when things cool off.
1. That lube isn't really lube. A man who is getting it on but suddenly realizes the need for lube might reach for whatever is handy, assuming a bottle of lube is not available - and sometimes that means he will try to use anything from shampoo or conditioner to lotion or cooking oils. Obviously these things are not meant for use as a sexual lubricant, and can lead to dry and cracked skin, irritation and more. Using lube designed specifically for the sexual purpose is always recommended.
2. The condom is too small. Though it is very important to always wrap it up before sexual activity, the wrapping itself might sometimes be the issue. Using a condom that is too small might lead to a little irritation in the heat of the moment, but a man can often ignore that for pursuit of pleasure. The aftermath is when things get rough, as the penis can develop small cuts, tears or even blisters from the use of a condom that is too tight. It pays to test out a variety of condoms to find the best fit.
3. There's been too much drinking. Sometimes a bit of inebriation can be a good thing, as it lowers inhibitions and can help both partners relax before the main event. But too much drinking can lead to serious problems, including trouble with getting it up or issues with stamina. But too much alcohol consumption can lead to even worse things, such as forgetting to use a condom or even suffering memory loss that leaves a man wondering just what happened the night before. Avoiding this is simple: don't drink too much before a bedtime romp.
4. Cleaning up is not a priority. There are plenty of bodily fluids sliding around during sexual activity, and much of that understandably winds up on a man's penis. From the natural lubrication that occurs during sex to the traces of spermicide in the condom, there can be several substances that linger. A man who doesn't clean off his penis after sex is asking for trouble, as those fluids can dry on the penis skin, leading to itching, irritation and redness, as well as a potential penis rash.
A man who pays attention to good penis care should always remember that caring for the penis extends to those moments when things are hot and heavy. Not only should a man be fully prepared for any sexual encounter, he should also be sure to take care to clean up well afterward. Maintaining good health should also include regular use of a high-quality penis health crème (health professionals recommend Man 1 Man Oil, which is clinically proven mild and safe for skin). Look for a crème that includes a multitude of vitamins and nutrients, such as vitamin A for odor-fighting properties, vitamin E to keep skin supple, and vitamin C to enhance sexual health. Amino acids such as L-arginine for blood vessel dilation and acetyl-L-carnitine for protection against peripheral nerve damage are also helpful. Combine it all in a Shea butter base and a man has the recipe for great penis health.
A kiss is an exalted affair; it’s about feeling each other’s cosmos, understanding each other’s emotional current without uttering words, for, the words would only sound ambiguous when emotions are intense. A kiss alone can speak volumes! But, do you think something more can be associated with it?
Yes, indeed kissing has several benefits than it may seem, sure, it feels great, but apart from that, it also lowers your blood pressure. It further releases epinephrine hormone into your blood which allows your heart to pump blood faster and hence reduces the level of bad cholesterol in the body.
Here is a list of reasons why you should kiss your partner more often:
In case you have a concern or query you can always consult an expert & get answers to your questions!
Asthma is a disease that intermittently affects the lungs. When it strikes, the lungs become inflamed and the airways narrow. The sufferer’s chest tightens, breathing becomes difficult or wheezy, and a persistent cough may develop – especially at night.
Asthma can be triggered by allergies or have its onset from a stressful period of life such as an infection, a grief, or even the birth of a baby. Thousands die from asthma each year. It is a serious disease that is increasing in frequency.
For children and adults alike, the struggle to breathe during an asthma attack can be a frightening experience. While conventional medications relieve many of the symptoms of asthma, they cannot cure it. With homoeopathy, it is a different story.
Causes:
Asthma is usually triggered by:
Homeopathy Treatment for Asthma-
When it comes to the treatment of Asthma, Homeopathy is much better at treating asthma. It is in stark contrast to allopathy, where inhalers and steroids are used to suppress inflammation. Homeopathic medicines do not aim at just reducing inflammation. They also intend to cure the basic cause of the recurrent inflammation. This way, not only the symptoms of asthma are treated but the incidence of asthmatic attacks is completely eliminated.
I have had patients coming to me who had been using inhalers or steroids for decades. They could not live without them. With a couple of months of Homeopathic treatment, they are able to live a disease free and medicine-free life. It is worthwhile to mention here that all Homeopathic remedies are natural and do not contain any chemicals. That is why these purely natural homeopathic remedies do not have any side effects at all. Some common homeopathic remedies for asthma include:
Other than these four remedies, some homeopathic remedies focus on other symptoms, such as:
NOTE: These remedies are extremely active and it is best to consult a homeopath before self-medicating.
In case you have a concern or query you can always consult an expert & get answers to your questions!
Seeing multiple strands of your hair leave your scalp with shampoo, on the floor while combing or on clothes later on is terrible. It gives a feeling of going bald if such a situation continues unabated, and that feeling is disturbing.
Hair fall has become a major problem for many nowadays. Often lack of proper care and knowledge about hair care products causes damage to hair and eventually hair fall. According to Ayurveda, hair fall is associated with body type that varies from person to person and also the stability of mind-body structure. Hair is considered as a byproduct of bone formation, as stated by Ayurveda. The tissues which are responsible for the development of bones are also responsible for hair growth.
Here are some ways through which Ayurveda addresses the problem of hair fall:
1. Dietary modifications: It is important to identify the lifestyle habits that cause hair fall problems. Consumption of excessive alcohol, meat, coffee, tea and smoking are some of these habits. Hair fall can also be aggravated by indulging in intake of too much greasy, spicy, oily, sour, fried and acidic foods.
Here are some ways suggested by Ayurveda to beat hair fall:
2. Ayurvedic herbs and medicated oil: bhringaraja, brahmi, amla, neem, ritha and ashwagandha are some ayurvedic herbs rich in essential nutrients that help in reducing stress and promoting hair growth. Different types of oils including coconut oil, brahmi oil, amla oil or mustard oil are useful in hair fall.
3. Yoga and meditation: Inverted asanas help in stimulating the blood flow to the head. Additionally, practicing deep breathing exercises to control anxiety, stress and keep the mind balanced is also advisable. If you wish to discuss about any specific problem, you can consult an Ayurveda.
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