In the United States, as a part of newborn screening, this test of 17-alpha-hydroxy-progesterone is carried out. Deficiency of 21-hydroxylase leads to the disorder of congenital adrenal hyperplasia (CAH). The disorder of congenital adrenal hyperplasia can be detected by the test of 17-alpha-hydroxy-progesterone. CAH and cortisol-related enzyme deficiencies are associated with each other. There are 90 % chances for the responsibility of a mutation in the 21-hydroxylase gene for causing CAH along with the detection by 17-OHP’s accumulation in the blood. The symptoms of CAH are different in infants, young girls and adult women and young boys and adult men.