Y-Chromosome infertility is a condition that affects the production of sperms, making it difficult or impossible for affected men to father children. In case of Y-Chromosome infertility, an affected man’s body may either produce no sperm cells known as azoospermia, a lesser number of sperm cells than usual known as oligospermia or sperm cells with an abnormal shape. In some cases, the patient may have mild to moderate oligospermia, in which case they can father children naturally eventually. In other cases assisted reproductive technologies are available to help the affected men. Y- Chromosome microdeletions are transmitted to all the male offsprings if the reproductive technologies are used. Y –Chromosome –Deletion test is ordered to determine the cause of infertility in men. It also helps predict the effectiveness of assisted reproductive technologies in men with specific Y chromosome microdeletions. The prevalent signs in order to prescribe this test include the duration of infertility, childhood urologic diseases or surgical procedures, any current or recent chronic illness, the social history of smoking and alcohol, spinal cord injury and family history.