AFB-MOTTO panel test is one of the most important tests when it comes to secure the public health. For a prompt management of public health, Rapid detection of Mycobacterium tuberculosis (MTB), especially multidrug-resistant strains is done. It is used to detect the TB infection that is happening due to one of the Mycobacteria present in the blood. It is done when a person is suffering from coughing; sneezing from a long time and even being medication the treatment is not working properly. It helps to know if the Acid-fast bacillus is present in the blood and then a new treatment is started.