Leptospirosis, the bacterial disease that affects humans and animals is a common condition in tropical and sub-tropical regions. It is caused by the bacteria Leptospira. The symptoms of leptospirosis can range from general; mild fever, headaches, vomiting, cough muscle pain and etc. to the other viral hemorrhagic, hepatitis, meningitis, and dengue’s. Animals are carrier of the bacterial infection. Vaccines for animals exist for certain type of Leptospira which ultimately decreases the risk of spread to humans. Hence, early diagnostic tests include testing a serum or blood sample serologically with a panel of different strains is vital.