Allergen, Individual-Grass pollen Bermuda Grass Durva (Cynodon Grass) Test

This test is used find out allergies caused due to pollen of Bermuda grass.

Bermudagrass occurs on waste places, roadsides, pastures and agricultural fields, in riparian areas adjacent to streams and marshes, and in the understory of open forests, orchards, and Pine (Pinus spp.) plantations. Bermuda grass pollen contains at least 12 IgE-binding proteins, 2 of these allergens have been described as major allergens since they reacted with IgE antibodies in more than 50% of sera in a study on 21 Bermuda grass-allergic individuals. Bermuda also contains species-specific allergen.

A diluted allergen is applied with a prick or a puncture on the surface of the skin. Intradermal. Using a very thin needle, a diluted allergen is injected immediately below the skin surface. After either type of test, the area of the skin is observed for about 15 minutes to see if a reaction develops. A raised, red, itchy bump indicates the presence of the allergy antibody when the person is exposed to specific allergens.

To detect whether the person have allergy from bermudagrass or not. Also used to identify numerous potential allergens. This includes airborne, food-related, and contact allergens.

2 ml of serum the watery (colorless part of the blood) is needed to this this, which has to be taken in a 3.5ml gold top tube container

Bermuda grass-pollen proteins were electrophoretically separated on polyacrylamide gels and transferred to nitrocellulose where IgE-binding components were detected by reaction with individual patient's serum and 125I-labeled anti human IgE. Seventeen pollen components, separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, bound IgE antibodies from a panel of 44 sera from allergic patients.