CD61 Test

CD61 is also called as integrin β3 and glycoprotein IIIa (gpIIIa), is a 90 kD type I important transmembrane glycoprotein. It is an adherent of the integrin family, relating to platelet gpIIb (CD41) to form a CD41/CD61 complex and with integrin αV (CD51) to form αV/β3 (CD51/CD61) integrin. CD41/CD61 is conveyed on platelets and megakaryocytes and plays a role in platelet activation and combination through interaction with fibrinogen, fibronectin, vWF, and other RGD- having adhesion molecules. CD51/CD61 is communicated on platelets, osteoclasts, fibroblasts, macrophages, and some tumor cells tangled in tumor metastasis and in adenovirus infection through binding to RGD motif in extracellular matrix proteins. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins collected from an alpha chain and a beta chain. A given chain may conglomerate with multiple partners ensuing in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to contribute to cell adhesion as well as cell-surface- mediated signing.

The antibody is purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

· Finds out the platelets, megakaryocytes and platelet thrombi
· Distinguish TTP (diffuse CD61+ platelet-rich thrombi) from DIC
· Count platelets in thrombocytopenic patients

Formalin-fixed paraffin-embedded tissue block; 1 H&E slide plus 3 unstained charged slides; fine needle aspirate in RPMI; bone marrow core fixed in formalin; bone marrow clot fixed in formalin.