IHC, Prolactin Test

Prolactin (PRL), also known as luteotropic hormone or luteotropin, is a protein that is best known for its role in enabling mammals, usually females, to produce milk. It is influential in over 300 separate processes in various vertebrates, including humans. Prolactin is secreted from the pituitary gland in response to eating, mating, estrogen treatment, ovulation and nursing. Prolactin is secreted in pulses in between these events. Prolactin plays an essential role in metabolism, regulation of the immune system and pancreatic development.

Prolactin is a peptide hormone primarily associated with lactation. It is synthesized and secreted by lactotrope cells in the adenohypophysis (anterior pituitary gland). It is also produced in other tissues including the breast and the decidua. Pituitary prolactin secretion is regulated by neuroendocrine neurons in the hypothalamus, most importantly by neurosecretory dopamine neurons of the arcuate nucleus, which inhibit prolactin secretion. Prolactin is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with lactotrope cells.

Tissue is carried out by needle biopsy procedure. Most needle biopsy procedure don’t require any preparation on your part. However, you may be asked to stop taking blood thickening medications like aspirin or warfarin before your biopsy. Depending on which part of your body biopsy needed and do not eat or drink before biopsy procedure. During procedure you will be given general anesthesia. You may receive intravenous sedation (IV) or general anaesthesia before the procedure. Your doctor may ask you fast the day before procedure Tell your doctor what medications you are in under Sometimes this would help doctor to make procedure easy.

When the antibodies bind to the antigen in the tissue sample, the enzyme or dye is activated, and the antigen can then be seen under a microscope. Immunohistochemistry is used to help diagnose diseases, such as cancer. It may also be used to help tell the difference between different types of cancer.

Tissue is Collected by needle biopsy The doctor uses a special needle to extract cells from suspicious area Generally, biopsy is done on tumours that your doctor can feel through your skin, such as breast lumps and enlarged lymph nodes. When combined with an imaging procedure, such as X-ray, needle biopsy can be used to collect cells from a suspicious area that can't be felt through the skin.