Neutrophil Gelatinase Associated Lipocalin Test

1. Anti-NGAL Antibody Coated Micro plate one micro plate surfaced with polyclonal anti-human NGAL antibody. A foil zipper bag should be wrapped around it and kept dry.
2. HRP Conjugated Anti-NGAL Antibody One vial in a preserved protein matrix.
3. The contents must be diluted with 870 mL of demineralized water and mixed well.
4. ELISA HRP Substrate One bottle of tetramethylbenzidine (TMB) with hydrogen peroxide.
5. ELISA Stop Solution One bottle containing 12 mL of stop solution.
6. Human NGAL Standards Six vials containing recombinant human NGAL in a lyophilized bovine serum-based matrix with a non-azide preservative.
7. Human NGAL Controls Two vials containing human NGAL in a lyophilized bovine serum based matrix with a non azide preservative
8. Tracer Antibody Diluent One bottle ready-to-use buffer. It should be used only for tracer antibody dilution
9. Calprotectin/NGAL Extraction Buffer One bottle of 20-fold concentrate. Before use the contents must be diluted of demineralized water and mixed well.

A test for early diagnosis of acute kidney damage has been developed by the National Institute for Clinical Excellence (NICE) The NGAL Test is a laboratory test used to determine the level of a chemical in human urine and blood. It identifies the primary spot of the damage. (proximal tubule, distal tubule, interstitial or vascular); It brings to notice the duration since kidney failure (AKI, chronic kidney disease or ‘acute-on-chronic’ kidney injury) Identifying kidney aetiologies(ischemia, toxins, sepsis or a combination) It forecasts the future and stratifies risk Supervises the response to AKI interventions role in expediting the drug development process

1. It is recommended that all the measures, control and unexplained samples should be assayed in duplicate.
2. The light sensitive reagents should be saved in former amber bottles.
3. It is advised to store any unused antibody coated strips in the foil zipper bag.
4. Precise technique and use of properly calibrated pipetting devices becomes essential.
5. Variations or changes in incubation times or temperatures other than the ones stated may affect the results.
6. All reagents should be mixed gently and thoroughly prior to use