Pax 8 Test

Pax-8 is a transcription factor involved in the development of kidney cell and thyroid. PAX-8 is a member of the paired box gene family. PAX is reported to be present in ovarian carcinomas. It can be shown to be expressed in high percentage of renal neoplasms. A tissue stain test which is known as Immunohistochemistry is used to know about the PAX-8. An analytical time involved in performing this test is one day and the laboratory test results can be made available in a span of two to three days. Only technical performance of the stain is required in this test.

As mentioned above the test required for the tissue PAX-8 is Immunohistochemistry and in this test, the only technical performance of the stain is required so pathologist’s interpretation is required or performed. The only precautionary thing required for this test is that its interpretation should be performed only with the context of patient’s previous clinical reports and the test should be diagnosed by a reputed pathologist. No significant cautionary methods have to be taken by the patient for the preparation of the test and there is no need of fasting as well. The patient can show up in their day to day attire and could maintain the regular diet as well.

PAX-8 is basically for the tissues containing the antigens and these antigens can be detected by performing the Immunohistochemistry test. Immunohistochemistry test is very useful for the detection of many diseases in patients. The major usefulness of Immunohistochemistry test is to detect the cancer disease in patients and to diagnosis it. The other major use of this test is to differentiate among the different type of cancer in the tissues. Apart from detecting and diagnosing cancer it also helps in identifying renal cell carcinomas, as well as papillary thyroid carcinomas and tumors of Mullerian origin.

Immunohistochemistry staining is the test required for the PAX-8 and it is a valuable tool in detecting the antigens in tissues. In order to perform the standard staining procedure, first, the tissue section has to be deparaffinized and then rehydrated before applying the primary antibody. Enzyme-conjugated secondary antibodies are then applied and the specific staining can be visualized after adding the enzyme-specific substrate. Occasionally, when weak or no staining is observed, an antigen 'unmasking' by enzyme digestion, may be required.