PIK3CA Test

P110 alpha, generally written as PIK3CA is one of the inhibiting cancer cells that are predominantly found in the internal cancer types like the ovarian cancer, breast cancer, gastric cancer, etc. The PIK3CA is a catalytic subunit of the PIK3 family which recently showed a significant fraction (25-30%) in the cells of colorectal cancer, glioblastomas, and small portions are observed in breast and lung cancer. Basically, the PIK3CA is an intracellular signaling pathway in the cell cycle that predominantly relates to the cellular quiescence, proliferation, and longevity. The PI3K activation activates AKT that localizes the plasma membrane. This in-turn affects many cycles and also this creates a pathway for growth promotion and proliferation over differentiation of the human body stem cells.

Formalin-fixed, paraffin-embedded tissues must be located on the patient’s body and marked so that there is no diversion in the area of sample extract. Patient should be informed in prior about the pros and cons about the test and complexity related to the same. Patient should be made free from all types of assumptions on the procedure of the test and sample collection. Well stained slides, needles and neat environment should be maintained prior to the test and sample collections. Very neat and clean cloth/cotton should be used to clean the peripherals of the sample extracted area and the stained area.

A typical cancer should be identified in the initial stages of its life cycle, the PKI3CA is one of the tests that creates a mere confidence in the patient’s mind about their disease and its diagnosis. The later stages of cure and the initial stages of prevention can be explained to the patient at the earliest at a easy note.

A broad needle with ample extraction compatibility should be selected. The exact tumor area/cancer affected area should be located over the patient’s body with the help naked hand and should be cleaned with alcohol before piercing the affected portion for the sample extract. 10-15 ml of the serum must be extracted from the tumor ball to the needle and collected in a neat tube which is then shipped for the actual examination area. While the sample is being extracted from the patient’s body makes sure that no blood enters into the sample. Once the sample is extracted, block the leakage of blood from the pierced portion by covering it with cloth or cotton.