Hi Sir, I am 21 years old bsc nursing student (male). I used to donate blood for needy at 6 months gap between each donation. Recently when I donated the blood they have detected my blood was hcv reactive. They said that they have done elisa twice and come to that decision. Immediately I have consulted medical Gastroenterology. He prescribed other tests like - hcv antibodies test, rt pcr (quantitative, genotyping for hcv. The results are given below 1) hcv antibody =0.03 (<1.0 not detected /non reactive). 2) hcv rna real time pcr= target not detected (<21-hcv rna below the detection limit of the assay or not present). 3) hepatitis c viral (hcv rna), genotype @ (pcr, sequencing: result=genotype not determined due to low viral load. My question is how elisa detected hcv in my blood where as all the above tests I have done in other lab (lalpath labs) could not detect hcv in that same blood. Is that possible or is it the mistake done by the transfusion people who.